Comparison on New Bone Formation Between Ovariectomized Rats and Normal Rats After Graft of Alloplastic bone material / 대한치과보철학회지

PURPOSE OF STUDY: To compare on bone formation between ovariectomized rats and normal rats after graft of alloplastic bone material. MATERIAL AND METHODS: Twenty female rats were used in this study. They were divided into two groups by each ten rats. One group was ovariectomized and grafted alloplastic bone material. Other group was non-ovariectomized and grafted alloplastic bone material. After a healing time of 4 weeks, the animals were sacrificed and decalcified preparations were routinely processed for histologic evaluations. RESULTS: Ovariectomy group was significantly lesser bone formation compared with non-ovariectomy group. CONCLUSIONS: Ovariectomy acts as a negative factor in new bone formation.

Study on the Expression of mRNA of Tumor Necrosis Factor-alpha and Interleukin-6 in the Cell Lines of Squamous Cell Carcinoma

The purpose of this study was to examine the mRNA levels of TNF-alpha and IL-6 in the cell lines of normal oral keratocyte and oral squamous cell carcinoma. Total RNA was extracted from these cell lines, observed under UV light, developed by radiographic films of PCR products via reverse transcriptase polymerase chain reaction (RT-PCR) amplication, and measured with densitometer. Each mRNA level of these cell lines divided by beta-actin mRNA level was compared to that of normal control group. The results were as follows : 1. Higher mRNA expression of TNF-alpha than IL-6 in the normal oral epithelial cell line. 2. In general, expression of mRNA of IL-6 appeared 3-4 times more in tumor cell lines than in control group. 3. mRNA expression of TNF-alpha showed variable expression in tumor cell lines, unlike normal cell line. 4. There are no special connections between differentiation of oral cancer cell lines and mRNA expression of TNF-alpha and IL-6. From the above results, expression of mRNA of IL-6 in the cell lines of squamous cell carcinoma used in this study has higher than the normal oral epithelial cell line, but there are no relationship between the differentiation of oral cancer cell lines and the expression of mRNA of TNF-alpha and IL-6.